mrna degradation การใช้

• There are two main requirements for mRNA degradation to take place:
• The recruitment of Argonaute proteins to targeted mRNA can induce mRNA degradation.
• The majority of miRNAs act in the cytoplasm and mediate mRNA degradation or translational arrest.
• RNA interference ( RNAi ) is a means of silencing genes by way of mRNA degradation.
• Because deadenylation is often the rate limiting step of mRNA degradation the enhancement of deadenylation increases mRNA turnover.
• RNase E is a key regulatory enzyme in the pathway of mRNA degradation in " Escherichia coli ".
• However, only few of the proteins reported to bind AREs were demonstrated to play a role in mRNA degradation.
• Morpholinos bind and block access to the target mRNA without requiring the activity of cellular proteins and without necessarily accelerating mRNA degradation.
• Occasionally, copurification of rRNA with degradosome is appreciated, which suggests that the complex may take part in rRNA and mRNA degradation.
• ARE-directed mRNA degradation is influenced by many exogenous factors, including phorbol esters, calcium ionophores, cytokines, and transcription inhibitors.
• These mechanisms include DNA methylation, RISC ) which is used to bind mRNA . This binding suppresses protein synthesis and sometimes initiates mRNA degradation.
• Detection of a premature stop codon triggers mRNA degradation by 5'decapping, 3'poly ( A ) tail removal, or endonucleolytic cleavage.
• Binding of a miRNA to a message can repress translation of that message and accelerate poly ( A ) tail removal, thereby hastening mRNA degradation.
• CCR4-NOT is a general transcription regulatory complex in yeast that is found to be associated with mRNA metabolism, transcription initiation, and mRNA degradation.
• It has been observed that the ability of a nonsense codon to cause mRNA degradation depends on its relative location to the downstream sequence element and associated proteins.
• All RODGI mutations which include frameshift, nonsense, and splice site mutations cause premature mRNA degradation or protein structure alteration that results in the lack of function of the protein.
• Mutation of any residues in the RNase H catalytic triad abolishes Slicer activity in human Argonaute protein Ago2, suggesting that the RNase H domain is directly responsible for target mRNA degradation.
• While degradation of miRNA-targeted mRNA is well documented, whether or not translational repression is accomplished through mRNA degradation, translational inhibition, or a combination of the two is hotly debated.
• In addition to mRNA degradation, uridylation is also thought to function in pre-microRNA maturation, with some group II pre-microRNA requiring 3'mono-uridylation for Dicer processing.
• However, in plants, the process has a much greater specificity to target mRNA and usually each miRNA only binds to one mRNA . A greater specificity means mRNA degradation is more likely to occur.